Homogenous, High-Throughput Assay to Measure Guanine Nucleotide Exchange Factor Activity

Guanine nucleotide exchange factors (GEFs) positively regulate Rho GTPases by accelerating GDP dissociation, allowing formation of the active, GTP-bound complex. This poster presents a simple, generic biochemical assay for measuring GEF activity based on GDP dissociation, developed by BellBrook Labs. The increase in GDP production is determined using the Transcreener^® GDP assay, which relies on selective fluorescence polarization (FP) based immunodetection of GDP.