How to Improve Your Chemiluminescent Western Blots

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method, chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is then captured and recorded. It is a highly sensitive assay and can detect femtograms of protein. Although it is only semi-quantitative, it is useful for detecting the presence or absence of a protein. Here are some tips to improve your chemiluminescent Western blots.