How to start a SPRi experiment

In the following application note, Horiba addresses several questions to consider before conducting a surface plasmon resonance imaging experiment. For example, multiple biochip sensors can be used and a number of functionalized surface chemistries are available to directly immobilize ligands of interest. Controls are another important consideration as refractive index changes and non-specific interactions are subtracted from signals. Running buffers can improve specific interactions and samples must be carefully prepared to avoid refractive index changes. Furthermore, the capacity for ligand regeneration is discussed, as well as how to evaluate the data produced by SPRi software.