Imaging dyes with large Stokes shifts on the Sapphire FL

Significant work has gone into developing fluorescent dyes with larger Stokes shifts for biological applications. Stokes shifts greater than 80 nm can minimize crosstalk between the excitation light and detection of emitted fluorescence during imaging, leading to better signal-to-noise ratios and better imaging. Explore how the Sapphire FL Biomolecular Imager provides the flexibility needed to image dyes with large Stokes shifts. Its modular excitation lasers and emission filters can be easily mixed and matched to achieve non-standard pairings compatible with specialty dyes. Azure Biosystems demonstrates the use of this mechanism on the Sapphire FL to image Western blots detected with antibodies labeled with MegaStokes dyes.