Immuno-oncology biomarker measurement with high sensitivity and speed

THis application note describes the successful implementation of the SimpleStep ELISA^® kits for detection of 3 relevant biomarkers: Programmed Death Ligand 1 (PD-L1), Interleukin-8 (IL-8), and Interferon Gamma (IFN-γ). Treatments targeting the Programmed Death 1 (PD-1) / PD-L1 pathway have seen successes due to the role of this pathway as an immune checkpoint. Beyond the immune regulator role, the expression level of PD-L1 in tumors, and in some instances, immune cells, has been correlated to the clinical outcome of blocking this pathway . In addition, both IL-8² and IFN-γ³ expression are shown to be modulated in response to PD-1/PD-L1-targeting treatments.