Improve Genome Editing Outcomes in Biologically Relevant Cell Models

The area of genome editing is rapidly growing and requires more advanced techniques to maximize its potential applications. Transcriptional activator-like effector nucleases (TALENs) and technology derived from clustered regularly interspaced short palindromic repeats (CRISPRs) allow precise cleavage of DNA at specific loci. However, the effectiveness of these tools is contingent upon the intrinsic properties of the locus of interest, efficient delivery, and the painstaking downstream processes of generating stable cell lines and knockout models to study the phenotypic effects of such genetic modifications. This application note assesses transfection of HepG2 and U2OS cell lines with TALEN and CRISPR vectors designed using GeneArt® Gene Synthesis services to target a specific locus.