Improved CRISPR/Cas9 Genome Editing in Hard­ to­ Transfect Mammalian Cells Using AAV

The use of CRISPR/Cas9 technology can be limited by delivery options for Cas9 and the single guide RNA (sgRNA). Transfection of cells with plasmids encoding Cas9 and sgRNA is the most commonly used method. However, many human cell types are considered hard­to­transfect, making plasmid­based delivery difficult. One alternate strategy for delivering CRISPR/Cas9 components to these cell types is viral transduction, learn more in this application note.