Improved Understanding of the Thermal Stability of a Monoclonal Antibody and its Modified Version by Use of DLS/Raman Spectroscopy

The combination of Dynamic Light Scattering (DLS) with Raman Spectroscopy provides the capability to investigate a wealth of chemical, structural, and physical parameters about biotherapeutic proteins under formulation conditions. This white paper demonstrates that by simultaneously monitoring both the colloidal (DLS) and conformational (Raman) stability of a template monoclonal antibody (mAb) and a modified monoclonal antibody (mmAb) provides significant insights into the mechanistic effects of thermal stress on the mAb and the mmAb.