Improving Fluorescence-Based Assay Performance Using Automated Digital Microscopy

One of the main limitations of fluorescence detection is background fluorescence, either from autofluorescence from endogenous cellular material, impurities from reagents added to the assay, unbound fluorescent conjugates or fluorogenic probes, or unreacted photoprotein. This study demonstrates three unique ways in which the incorporation of imaging, in addition to cellular analysis, allow for a more accurate analysis of the event taking place within the microplate well, and improve assay performance.