In-Cell Western™ Assay Quality Assessment Using Z’-Factor

In this application note Z’-factor experiments are performed on one or more ICW assay plates containing replicate wells designated for background subtraction, negative control samples, and positive control samples. During ICW assay optimization, it is important to assess the overall quality and reliability of the assay. The Z’-factor statistic provides a way to evaluate whether or not assay conditions (reagents, protocols, instrumentation, kinetics, and other conditions not directly related to the test compounds) are optimized. Z’-factor is a dimensionless value that represents both the variability and the dynamic range between two sets of sample control data.