In Vitro Study of Vascular Microenvironment During Atherosclerosis Development

This application note investigates the role of different stimuli recognized to play an important part in atherosclerosis development (chemokines, cytokines, oxidized lipoproteins). Using Cellix’s biochips and Mirus pumping system, THP-1 adhesion to VCAM-1 and HUVECs; PBMC adhesion to Fractalkine and MCP-1; and THP-1 adhesion to oxLDL treated HUVECs is investigated; all under physiologically relevant shear stress. These in vitro studies, closely mimicking the human in vivo vascular microenvironment, represent a useful model to dissect different mechanisms involved in atherogenesis.