Incorporation of Cytation™ Cell Imaging Multi-Mode Readers and Gen5™ Data Analysis Software to Create Deconvoluted, Stacked Images of 3D Cellular Structures

Three-dimensional (3D) cellular models have the potential to become a fundamental research tool in cell biology and histology slides containing tissue samples mounted onto microscope slides, continue to be an important research and diagnostic method in the clinic and laboratory. These multi-dimensional cell structures present complications for optical microscopy due to their thickness in the z-axis. This application note presents a method to capture and project multiple single z-plane images using digital widefield microscopy.