Inhibition of MicroRNA Sensitizes 3D Breast Cancer Microtissues to Radiation Therapy

3D tumor microtissues are widely accepted as being more physiologically relevant than conventional 2D monolayer cell cultures, and can therefore help generate more predictive data. This case study describes an investigation by researchers within the Personalized Radiation Therapy Group into the diosensitizing potential of miR-21 down-regulation, using T47D breast cancer cells and T47D 3D breast cancer microtissues. Dr. Nataša Anastasov explains that by using the Operetta® High Content Imaging System, the growth of microtissues can be easily monitored by capturing the whole well of the Gravity TRAP^TM plate from InSphero.