IntelliQube® Real-Time Quantitative PCR Performance Characterization

The IntelliQube from Douglas Scientific is a fully integrated laboratory instrument that combines liquid handling with real-time quantitative PCR (qPCR) analysis in miniaturized reaction volumes. This application note reports on the performance of the IntelliQube. The performance characteristics of this innovative instrument were determined by a series of PCR-based experiments that interrogated PCR uniformity, resolution, and dynamic range. Additional experiments were performed to demonstrate the utilization of all five fluorescence channels, as well as a multiplex reaction with FAM and HEX reporters.