Intercalating Fluorescence Dyes: A Comparison of Different Kits Using the qTOWER from Analytik Jena

Using intercalating dyes for quantification assays is one of the most common and popular real-time PCR applications. SYBR® Green or EvaGreen® for example are easy to use. Thus a wide variety of ready-to-use master mixes are available to simplify workflows as much as possible. Analytik Jena’s standard real-time thermal cycler qTOWER 2.0/2.2 was used to compare the performance of those mixes.