Investigation of the Stereoselectivity of an Anti-Amino Acid Antibody Utilizing Tryptophan Fluorescence

This aim of the study presented in this application note was to test if tryptophan fluorescence can be used to determine the affinity of an anti-D-amino acid antibody toward a variety of standard and non-standard amino acids. The FLUOstar plate reader from BMG LABTECH enabled the measurement of tryptophan fluorescence with high sensitivity and good signal-to-noise ratios. The excitation of appropriate proteins at 280 nm and measurement of fluorescence emission at 350 nm can be employed to investigate protein-ligand interactions and deduce, for example, binding affinities.