Ion Mobility-Enabled Data-Dependent Experiments Distinguishing Co-Eluting Isomeric Metabolites Using an IMS-QTof Mass Spectrometer

LC-MS/MS using data-dependent acquisition (DDA) is widely used for characterization of metabolites in drug metabolism studies. In this application note, nefazodone was incubated with rat hepatocytes and analyzed using both DDA and IMS-enabled DDA and co-eluting metabolites were characterized.