Comprehensive isotope ratio analysis of amino acids using Orbitrap Exploris Isotope Solutions

This technical note highlights the use of Thermo Scientific^™ Orbitrap Exploris^™ Isotope Solutions for precise isotope ratio analysis of amino acids, focusing on glutamic acid as a model compound. Glutamate plays a key role in metabolism, neurotransmission, and nitrogen excretion. The advanced Orbitrap^™ technology enables simultaneous carbon, nitrogen, oxygen, and hydrogen isotope ratio analysis from intact molecular ions by a single analysis, offering valuable insights into biochemical processes and metabolic pathways.