Klearkall Master Mix for High-Throughput Genotyping on Crude Plant Samples to Enable Precision Breeding

SNP genotyping is a powerful tool to accelerate breeding programmes for agricultural crop improvement. The crude DNA extracts often used in such high-throughput genotyping projects are rapid and cost-effective to prepare, but can pose significant challenges to downstream analysis due to carry-over of endogenous PCR inhibitors from the original plant material. This application note demonstrates high-throughput end-point genotyping using the KlearKall Master mix, an optimized 2X PCR reaction mix.