Label-Free Phenotype MicroArrays™ Analysis of Cellular Energetics and Apoptotic Activity Using Microplate Reading and Phase Contrast Imaging

Phenotype MicroArrays™ is a unique label-free multiplexing technology for screening cell-based energetics that describe a variety of metabolically related phenotypes in a target cell model. This application note demonstrates a method to combine cellular metabolic Phenotype MicroArrays™ and automated, digital widefield phase contrast microscopy to screen differential nutritional pathway usage of test cells, in addition to potential pathway interruption by an apoptotic compound.