<i>In Vitro</i> Cleavage Efficiency of sgRNAs Correlates with Functional Genome Editing in Target Cells

The Guide­it sgRNA Screening Kit is a complete system for predicting the cleavage efficacy of sgRNAs in vitro, prior to use for genome editing in cells. With this kit, a template containing an sgRNA­target site is created by PCR; then the test sgRNA and recombinant Cas9 nuclease are added. The efficiency of Cas9­ mediated cleavage can be measured by agarose gel electrophoresis.