Lipid Cubic Phase: Drop Setting, Condition Screening and Imaging

Integral membrane proteins are arguably the most important class of proteins in terms of their role in cellular interactions and their associated significance in disease pathways. Lipidic Cubic Phase (LCP) has emerged as an effective medium for crystallizing membrane proteins. The current pace of adoption of LCP-based methods has increased substantially, in spite of the steep learning curve associated with their implementation. This application note will discuss the three major steps of LCP crystallization and how automation technology is significantly facilitating the use of this powerful methodology.