Long-term live cell visualization and quantification of neuronal activity

In this application note, Sartorius presents data to support the use of the the IncuCyte S3 for Neuroscience to characterize and refine different neuronal phenotypes and model their function in vitro. Here, Sartorius explains how this single live-cell imaging platform allows users to assess calcium flux kinetics and continuously monitor the morphology of neuronal populations, using non-perturbing reagents, validated protocols that are cell-sparing, and a built-in, guided interface for non-experts, as provided by the IncuCyte S3 Neuronal Activity Analysis Software Module.