Lysing Micro-Organisms with the Vk05 15 Ml Precellys® Lysing Kit

Microbes such as yeast or bacteria are difficult to lyse due to their thick cell wall, which makes nucleic acid extraction difficult. Gram positive bacteria for example, have a cell envelope comprised of the plasma membrane and a thick cell wall containing peptidoglycans. This application note demonstrates how the Precellys^® homogenizers offer an alternative and more effective lysing solution for DNA and RNA extraction to be used for molecular biology research, such as sequencing, PCR or cloning.