Measurement of Red Fluorescent Dyes for Improved Multiplexing Capabilities and Cellular Assays on the CLARIOstar Plus

This application note details the use of the CLARIOstar Plus in high-throughput red fluorescent (HTRF) screening. Due to increased use of cell-based assays and multiplexing, fluorophores emitting in the red are on the rise. Here, the suitability of the CLARIOstar Plus to detect such dyes was demonstrated. It displayed high sensitivity with a low LOD for AlexaFluor647 (0.8 pM) and significantly improved HTRF measurements.