Measuring genetics, 5-mC and 5-hmC at single-base resolution

The duet multiomics solution evoC is an innovative sequencing technology that simultaneously resolves the four genetic bases and distinguishes between cytosine modifications 5mC and 5hmC (six-base calling). By integrating genetic and epigenetic data at high accuracy, evoC enables deeper insights into gene expression, chromatin accessibility, and enhancer states. Using mouse embryonic stem cells, this method reveals the dynamic interplay between DNA modifications and transcriptional regulation.