Measuring the Particle Size and Concentration of Adeno-Associated Virus using Nanoparticle Tracking Analysis

This whitepaper describes the characterization of gold-labeled Adeno-Associated Virus (AAV) and other small viruses by Nanoparticle Tracking Analysis (NTA). It details the method of gold-labelling the virus particles followed by using the NanoSight from Malvern Instruments to utilizes the properties of both light scattering and Brownian motion to obtain the particle size distribution of virus in liquid suspension. The paper concludes that this method can directly measure AAV particle concentration in a matter of hours.