Meeting Ultra-High Throughput Imaging Challenges

This application note describes the use of cell based assays, using high content imaging in drug screening or toxicity testing screening. Screening in 1536 well plates can often be time consuming, but with the large field-of-view enabled with the ImageXpress®Micro XLS System and improvements in MetaXpress® 5 High Content Image Acquisition and Analysis software, whole well images of an entire plate can be acquired at 3 wavelengths in 15 minutes and analyzed using either the standard analysis software or accelerated with MetaXpress® PowerCore™ parallel processing software. This experiment demonstrates equivalent results in an antibody binding assay using beads, whether utilizing standard imaging methods or a new ultra-high throughput workflow.