Metacomplex Formation & Binding Affinity of Multivalent Binding Partners

This application note describes the use of (CG-MALS) to investigate formation of a metacomplex between streptavidin (SA), and a bivalent antibody (Ab) in solution. A Calypso® II connected to an inline UV/Vis concentration detector and DAWN® HELEOS® MALS detector were used to create automated composition gradients. This article highlights the uniquie suitability of CG-MALS for investigating complex systems of macromolecules in solution.