Method Development Considerations for Paper Spray Mass Spectrometry - Direct Ionization Technique for Physiological Fluid Analysis

Sports doping in both humans and animals is a widespread problem. Paper spray is a direct ionization technique that simplifies the mass spectrometric analysis of compounds from physiological fluids, making it an attractive method for clinical research and forensic toxicology applications. In this work, methamphetamine and other stimulant drugs were spiked in horse urine to demonstrate the potential of the technique to monitor sport doping in horse racing. Paper spray ionization presents challenges for mass spectrometric analysis due to the lack of specimen matrix clean-up and lack of chromatography. This poster outlines a method development approach using paper spray Ionization mass spectrometry with this in mind.