Micro-Volume Determination of Labeling Efficiency Using Spectral Scanning Analysis in a Standard Microplate Reader

The use of labeled reagents in a variety of experimental methods such as immunofluorescence, TR-FRET, and ELISA has been shown to provide substantial improvements in assay sensitivity while allowing sample and reagent conservation in a high-throughput context. This application note describes the analysis of protein-antibody conjugates labeled with biotin or digoxigenin using micro-volume spectral-scanning analysis in a microplate reader.