MicroRNA Expression Profiling of Individual Rat Hypothalamic Nuclei

This application note demonstrates the acquisition of individual miRNA expression profiles from single hypothalamic nuclei provided by cDNA library sequencing using a GAIIX machine. MicroRNAs (miRNAs) finely tune messenger RNA(mRNA) expression. As the brain is a highly heterogeneous tissue, physiologically relevant miRNA expression profiling greatly benefits from sampling brain regions or nuclei.