Middle-Down Analysis of Monoclonal Antibody Using Nano-Flow Liquid Chromatography and a Novel Tribrid Orbitrap Mass Spectrometer

This application note demonstrates sensitive analysis of monoclonal antibody using a middle-down approach on a novel Thermo Scientific™ Orbitrap Fusion™ Tribrid™ mass spectrometer. The method combines nano-flow liquid chromatography and complementary higher-energy collisional dissociation (HCD) and electron transfer dissociation (ETD) fragmentation on an Orbitrap Fusion Mass Spectrometer.