Modular DNA Assembly of PIK3CA Using Acoustic Liquid Transfer in Nanoliter Volumes

In this study, NEBuilder^® HiFi was combined with the 25 nL increment dispensing Labcyte Echo^® 525 Liquid Handler to assemble a modular oncogene construct encoding phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) over five modular pieces. The goal of this experiment was to utilize the Echo 525 Liquid Handler in assembling five, five-piece assemblies by substituting modular DNA pieces at the nanoliter scale. The Labcyte Echo Liquid Handler enables lower-cost methods and workflows to produce high-quality synthetic DNA constructs which expands design-based testing with higher throughput and affords the scientist a broader biological landscape to interrogate.