Monitoring Cell Cycle Progression Using Fluorescence Microscopy

The concept of the cell cycle is used to describe the repetition of events that lead to the duplication of cellular organelles during cellular replication. Compounds that affect cellular transition through the cell cycle have the potential to be effective anti-cancer agents. Here we describe the use of digital widefield fluorescence microscopy in conjunction with different fluorescence assays to monitor cell cycle progression in live and fixed human tissue culture cells.