Monitoring Cytotoxicity on the SpectraMax i3 Multi-Mode Microplate Platform with SpectraMax MiniMax Imaging Cytometer

The SpectraMax® i3 Multi-Mode Detection Platform allows users to measure apoptosis and cell viability on a well-by-well or cell-by-cell basis, as well as control assay quality by monitoring cells’ appearance. This application note demonstrate how a more complete assessment of cell health can be performed using a combination of fluorescence imaging and luminescence-based assays. Fluorescent imaging of caspase-3/7 activity, an essential event of apoptosis, was combined with a luminescent ATP assay to monitor cell viability.