Monitoring Receptor Mediated Changes in Calcium Levels Using the FlexStation Benchtop Multi-Mode Microplate Reader

The FlexStation® Benchtop Multi-Mode Microplate Reader from Molecular Devices integrates sensitive optics, fluid transfer and temperature control making it ideal for kinetic, cell-based fluorometric assays, such as the measurement of intracellular calcium, membrane potential and intracellular pH. In this application note ATP caused a concentration-dependent increase in intracellular calcium levels in CHO cells endogenously expressing the P2Y₂-receptor. It is demonstrated that the FlexStation system can be used to generate reliable, reproducible data for calcium mobilization assays used to monitor G_q-coupled GPCRs.