Multiomic 6-base data from cell-free DNA enhances the performance of liquid biopsy classifiers

Early cancer detection has the potential to significantly improve treatment outcomes and survival rates. This study investigates the roles of 5- methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) as biomarkers for early-stage colorectal cancer (CRC) detection in cell-free DNA (cfDNA), using duet evoC for whole genome 6-base sequencing. The results support the hypothesis that distinguishing between 5mC and 5hmC can improve the sensitivity of liquid biopsy tests for early cancer detection.