Neural outgrowth analysis in 3D human brain micro-tissues for compound screening applications

Organotypic, three-dimensional (3D) cultures, such as brain organoids, offer higher in vivo relevance compared to traditional 2D cultures for studying neurological development and disease. In this application note, Molecular Devices highlights RealBrain^® micro-tissues by Tessara Therapeutics which provide greater physiological relevance and scalability for analyzing 3D neurite outgrowth. In a study using RealBrain micro-tissues, compounds affecting neurite outgrowth were imaged using high-content imaging and analyzed with MetaXpress^® software.