Neurosphere Formation, Differentiation, and Migration of Human Neural Stem Cells Cultured in Corning® Spheroid Microplates

Neurosphere formation of human neural stem cells (hNSCs) is a widely used in vitro culture system and valuable model to study neurogenesis and neural development. This system allows for three-dimensional (3D) expansion of hNSCs within a more physiologically relevant microenvironment. In this study, Corning spheroid microplates were used for neurosphere formation, proliferation, and migration of hNSCs in an easy-to-use format that is amenable to high throughput screening.