Neurosphere Formation, Migration, and Differentiation of Human Neural Stem Cells Cultured in Corning® Spheroid Microplates

In this study, the spheroid microplates allow for NSC culture of uniform and directed neurosphere size over the course of 96 hours, throughout which multipotentcy is maintained, as assessed through Nestin and Sox2 marker expression. Analysis of spheroid size and quantification of migration was accomplished with the BioTek Cytation™ 5 Cell Imaging Multi-Mode Reader.