Non-Invasive Analysis of Drug Sensitivity in Homo and Heterotypic Multicellular Tumor Spheroids

To gain full benefit of more organotypic tumor culture systems, appropriate assays which enable high-throughput analysis over time without disrupting the tissues have to be adopted. Read outs for co-culture systems which allow the discrimination of a drug effect on the cancer cell and stroma environment will be a tremendous improvement when using complex 3D models. This poster reports the development of microtissue models harboring fluorescence reporter for non-disruptive drug sensitivity testing of homotypic and heterotypic colon cancer spheroids.