Novel Instrument for Electrophysiological Guidance of Nanoliter Injections in the Brain

In this poster, the Nanoject II was successfully modified to record extracellular neural activity. The Nanoject II uses a direct drive, a stainless steel piston inside a fluid filled pipette, and can inject as little as 2.3nl. The experiments tested this new instrument for electrophysiological localization of the gustatory responsive area of the parabrachial nucleus. The effects of small excitotoxic lesions suggest a functional topography within the gustatory parabrachial nucleus.