Off-target effects: Disturbing the silence of RNA interference (RNAi)

Off-target effects occur when an siRNA is processed by the RNA-Induced Silencing Complex (RISC) and down-regulates unintended targets. As these changes in gene expression can lead to measurable phenotypes (such as false positives) it is of great importance to understand the mechanism behind off-targeting so that strategies can be developed to minimize their effects. Download this application note by Horizon Discovery to find out what can decrease off-target effects and therefore allow for better gene silencing.