Optimization of a Label-free Localized Surface Plasmon Resonance Assay on a Spectral Scanning Microplate Reader

This note describes how to perform and optimize a SoPRano measurement on two Thermo Scientific microplate spectrophotometers. Detection of the interaction of human Serum Albumin (hSA) conjugated to gold nanoparticles and anti-hSA monoclonal antibody is used as a model assay.