Optimization of the PowerSoil®-htp 96 Well Soil DNA Isolation Kit on epMotion® 5075 VAC

MO BIO Laboratories Inc. developed the unique PowerSoil-htp 96 Well Soil DNA Isolation Kit for isolating genomic DNA from environmental samples while also removing PCR inhibitors. This kit was optimized on the epMotion 5075 VAC automated pipetting system allowing greater pipetting accuracy while offering a more efficient workflow. DNA was extracted from six soil types from multiple sites in central and southwest Michigan using the PowerSoil-htp 96 Well Soil DNA Isolation Kit producing varying yields of DNA sufficient for subsequent testing and amplification of the DNA and showing little evidence of PCR inhibition from common inhibitors such as humic acids. Results demonstrated that DNA yields on the robot were similar to those obtained using individual silica spin filters.