Optimizing Settings to Validate the BMG LABTECH CLARIOstar HTS Microplate readers with the Transcreener TR-FRET Assays

Transcreener® is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes. Many of these enzymes catalyze the covalent regulatory reactions that are central to cell signaling; e.g., phosphorylati on, methylation, and are of high interest as therapeutic targets. This application protocol describes the optimal instrument parameters used to validate the CLARIOstar® Microplate Reader with the Transcreener ADP² TR-FRET and Transcreener AMP²/GMP² TR-FRET assays from BellBrook Labs.