Optimizing the hERG Assay on the PatchXpress 7000A System for Compounds that Demonstrate Non-specific Binding

In this application poster eight compounds, astemizole, pimozide, dofetilide, cisapride, terfenadine, flunarizine, quinidine and imipramine were used to evaluate the effect of different assay conditions to overcome the effects of non-specific binding in a hERG assay on the PatchXpress 7000A system. To achieve the best IC50 values, care had to be taken in all phases of compound preparation. Compound binding to plastic container was minimized. Buffers used for the assay also had to be optimized.