PacBio SMRTbell Prep 3.0

The PacBio^® SMRTbell^® Prep 3.0 for MIRO CANVAS^® NGS prep system provides PCR-free library preparation for long DNA fragments for use in PacBio sequencing. INTEGRA Biosciences details a list of required materials, equipment, and consumables, along with step-by-step procedures for preparing reagents, loading the MIRO Cartridge, starting the instrument run, and recovering the sample. Additionally, INTEGRA addresses frequently asked questions regarding Dropgloss, pipette tip sizes, residual reagent, bubbles in pipette tips, regurgitation during cartridge loading, and the specific dilution of AMPure^® PB beads.