Peptide-Antibody Interaction Specificity Using SPRi

This application note demonstrates the relevance of using SPRi technology for research into peptide-antibody interactions. First, streptavidin grafting is performed by using GenOptics pyrrole electro-polymerization protocol. A mix of pyrrole and pyrrolated streptavidin is co-polymerized on the gold layer of a glass prism. Biotinylated peptides are then injected in the detection cell and create a homogenous peptide layer, due to the strong affinity between streptavidin and biotin. Use of SPRi technology allows real-time studies of peptide-antibody interactions on a large number of spots analyzed in parallel.